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Qian Liu, Workshop 500: Bioconductor toolchain for development of reproducible pipelines in CWL

Qian Liu
Assistant Professor в Roswell Park Comprehensive Cancer Center
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500: Bioconductor toolchain for usage and development of reproducible bioinformatics pipelines in CWL

Qian Liu (Roswell Park Comprehensive Cancer Center)

Qiang Hu (Roswell Park Comprehensive Cancer Center)

5:00 PM - 5:55 PM EDT on Wednesday, 29 July

WORKSHOP

This workshop introduces the Bioconductor toolchain for usage and development of reproducible bioinformatics pipelines using packages of Rcwl and RcwlPipelines. The Common Workflow Language (CWL) is an open standard for development of data analysis workflows that is portable and scalable across different tools and working environments. Rcwl provides a simple way to wrap command line tools and build CWL data analysis pipelines programmatically within R. It increases the ease of development, usage, and maintenance of CWL pipelines, and furthermore offers higher performance by intuitively supporting parallel work on high performance computing (HPC). hundreds of pre-built bioinformatics pipelines in CWL are included in RcwlPipelines. The tools and pipelines are highly modularized for easy customization of complex bioinformatics analysis. An scRNA-seq pipeline using STARsolo for alignment and quantification, DropletUtils for filtering raw gene-barcode matrix, fastqc and multiqc for reads quality control will demonstrate the typical use case of these two packages. More details for usage and examples are available on Rcwl website: https://hubentu.github.io/Rcwl/.

Moderator: Nitesh Turaga

О спикерах

Qian Liu
Assistant Professor в Roswell Park Comprehensive Cancer Center
Qiang Hu
Postdoc в Roswell Park Comprehensive Cancer Center

I am an Assistant Professor with 8+ years of experience in data mining, with an application in the area of bioinformatics and statistical genomics. I have contributed 10+ peer reviewed papers with some additional conference presentations. My skills include high-throughput data analysis, including next generation sequencing data analysis and survival analysis, as well as parallel computing with daily use of R and shell scripting. I have an interdisciplinary background in pharmaceutics, molecular biology/biochemistry, and statistics, which enables me to have solid understanding of the statistical principles behind current best practices in the high-throughput data analysis, as well as a solid understanding of the relevant concepts in cancer biology and genetics.

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Hey, good afternoon everyone. Welcome to my workshop. My name is Chen Lu. And the first, I want to thank the planning team for when I sing this fantastic virtual conference and thanking attached for moderating discussion. I would like to give you several links over here. You see the link for the top of this workshop and the doctor have and you have the link all over here for Rodney McLeod. The goddamn mom called and packaged on website so you can click on these for the Call these tennis shoes that are connected to change

and put in your email. Address in King submitted, use the username and password over here to launch the workshop. Baby are still do, and they'll welcome to about the 2020. Okay. So Isis loading, I have pasty the link of the webpage for this Workshop. If you click on this link over here and you can see the the webpage for this workshop with some Workshop descriptions and the dog construction. Also if you have dug her installed on your computer already, you can just copy and paste these

two lines of code to your terminal so that you can run this image as an alternative to the cob instant So if you click on the workshop, over here, you see the full content of the rock shop, I'll be presenting today. And also this Workshop is meaning of all the usage of the pre are already, previews, are stubborn Oil Tools and pipelines. And if you're more interested in the development of your own child, choose you can click on the article spell c2020, our stuff out for developers. There's another one that's

ready for you to use. Okay, let me go back to the possible. And I've you be present during several slice first for the introduction and the channel to be happy to answer questions in the end of the week, or if you have extra time. So I'll start with the packages are sick. A common challenge that is faced by many both of my addictions, they reproduce ability of your pipeline, so it could be long. I could be trying to reproduce, you're a collaborator pipeline that

is developed a different computer and studying or you're trying to produce some new method that has just published. People has actually tried to calm by the time for reproducing them new message. So they has estimated about 280 hours or no life with many more than from that takes the expertise. So used to be far more skilled as they have you met, you slice of, but still the time is really alarm for reproducibility. So there are several aspects involved this challenges for space to stop to write appendix.

We need space. If they can stay the worshippers of the software for specific analysis. Also, the connections between stats could be very time-consuming, especially if you're my daily. Monitoring your stats in your cup fine. And we do you need a standardized description language to connect steps with it, including I'll post them. People could use our time difference, Computing environments, they can work locally with different operating system work on a PC with different job managing. And they can also work on the columns for big

data on different platforms. So for a reproduce reproduce environments, The which language would present a solution for those challenges. So there's not a popular one such as this, make a python workflow manager, the workload description language from broad Institute and I'm slow that they have driven. Invitational pack my tools in a way that makes them portable and scalable, a crossover, radio. Try, and Hardware environment from work stations to claster cloud and a high

performance Computing environments. It has been very widely used in the sentence of science that has been for my medical imaging machine learning and it has been implemented on my caller ID on platforms such as Galaxy in Cromwell. So he's so many advantages of using the pipeline workflow language. There are also many challenges in implementing them into your own data analysis, and learning curve is now Triple A Or are implementing see that route because it requires a level of expertise that is far beyond the capabilities of

French scientist in the even very hard for to implement for a skilled data analyst at mine. It's like the connection to the downstream data analysis that I haven't done with our and that conducts her again. So, we should I buy interoperability with cheese and pepperoni, and the public are the steps in different sizes. So we have devolved to our package call Darcy wll. It is our interface to ride Citadel scrapes. So we are intended to provide essential function on a standardized, the development of cities,

and to promote the motorized easier, sharing of established Popeye's or a critical steps. So, it provides three sets of main categories of functions. First dates that conversation. We can use these two functions to construct a c. We can execute your pretty fine to see that. I've had clients in local computer, using the high-performance Computing or with a shiny app and we have also enabled visualization function capacity to make the available for you easier for your communication with collaborators. Especially

I was just interrupt this one question which popped up may be useful for people to follow for the rest of the workshop to bring is asking why you chose cwl and not wdl or other word for languages. I guess that's what's most relevant. And there's also a follower of what are the main differences between wdl and cwl? I've you maybe I can't answer this question in the end of Okay, yeah, I said there. There's now there's no our interfaces for the others accountable. So we have started used to sit around maybe if they're more users for the other papaya languages, we can

consider adding a interface that is not like comparing the different topic of it. So if you make a language so you can pick me up on the way. You'll see. And also to see that has structure you say stuff like that structure language are similar to The llamo which argues that I sound like the higher if you're being. So it is very easy are for our users to understand. Thanks, I'll continue with my slice. So here is show sound simplified pipeline for the single-cell. RNA sequencing data

processing, the tool called a star solo for the realignment, and droplets you to use for them getting a high-quality account when she. So typically three steps video involved, if you want to build your own to one run a pipeline in, are the first two staff is to be able to see the real chill. So this is 4 divided /. Something if you want to rub your own to you because we already have like prey viewed more than a hundred previews to us and pipelines and they are

free to use. Basically, you need to fill a construction are objects of the tool in are using the CW Prime. Do you need them for the basic amount of the command line to be called a star and new? Put the input parameters and put out. Pope Francis silver in both printers, you need to assign a name for it. Like I'm training bird ID, you need to specify the file tab for this Prime internet. You need to buy the prefix if you're using it in with my life. So we got to Bounce by sparky on the last reference and I'll put bombs to go out like

two adults. One is the file, the dance of the alignment AmStar solo and why is that so loud that out your X-ray, which Saves The Raw? Count Matrix from Star solo. Dr. All restart. Okay, so these are just feel, don't call that you show you some real scripts later, if you're in the lab demo and the Second Step used to connect this to us in. So now we have the tool called star solo and presumably, we already have the tool called the job that you to use Define. Now

he wants, he can I please tutus and first, we need to use the time to initiate the Sci-Fi by assigning the input and output that we need to use the step function to specify that the two steps in this pipeline. The four steps that Easter starts so low. Each of you. I'm here and you need to run them till we have, just be finding the step one cuz star solo, and the inputs for this that and the second staff training, and the RCW about you. Here and the input will be impulsive. This Chaplet YouTube From the Star solo

by reading the house in this folder. And the next is to just simply collect these tools together into a pipeline that using the pot sign. Now, we have a sleep at writing. He's just ask you this time. I see you just busy by the name of your pad, thai in our, and I'll put your X-ray, or where do you want to save your outfit files and The Inkwell too late. You just assign the the weather for the info spouse. The absolute path over here, for this Ice Cube's house, and the past to your reference human genome.

I'll get you more details of this papaya in the lab demo. We also have our package called RCW. Appliance, it is a collection of our cwlt with and Piper counseling. We have a hundred 3 2026 Advanced pre built and tested any conversations wide range of this analysis, including we have some specific set of Ty panitz Define such as the only one teaching prediction, Pizza Pipeline has integration money issues, including the DNA sequencing alignment over here and re-read. Constipation number here in mutation where

in calling for the humor, normal pairs in production still buy running this whole pad fine. I will show you how to use it. You will get the results directly like for the ranks and do antigens. Just assign riders for this this yellow blocks. These are the raw files as input. These projects out, RCW, supplies, our Focus to Wallace a community-driven platform, where open source, open development and open review of best practice C, W album from attics, I find a cheap lab races in contributions

from the community to Admiral specialized instead of two of them have lines to the collection. And by using those existing the previous show us some Popeyes, provide a three-car functions to facilitate the usage and which we help users to sync. The current are stubborn, a recipe to search for interested to a Popeyes using Huber's and Soros. The tools from Popeyes. I mean, she's into your art station. Okay, so if you have already started your copies tensor, the dog her. Have you used the dog Cremation

by Play the song. This link over here. I've you, okay, I have already opened it. So it's up to you to login. You need to use the Art Studio. as the username and the Falcons answer at the password, if you are using the docker image, but you fear using the cloud You may be using our studio as username. And the welcome - 2 - 2020 as the username. You seen the doctor here. So, We are prompt to the art studio. You can click on the weenie ass over here, and click on the first vignette.

And you may be having this available here. So for this Workshop will require some basic knowledge of using our and that conducts, our packages and basic familiarity with running a command line tools. No prior experience with it said that I always necessary. The chili I have just give you an overview of the bathroom at least stop by and see. How do you show you how to use those car functions to update search? And load the to Oso tap lines and how to use the Single Cell in Maxton tool alignment tool and

also the vine that combine, the Stars still on the shelf that YouTube how to write mine to Laura, customize your own pipeline. You can go to the other rainiest year later. First. Major deals that they had resources. Okay, let's go back to you. The home directory and let me have prepared, all the fast needed in his Workshop by initiating for this Workshop. You should already have it available in the file path over here. So clicked on the East and the tested. So these are all the files that we going to be need for this left. I'm old. And that if you

just a chunk of coal do you should already have the test data on the receipt. From tonight's genomics, with relatively small amount of RNA, sequencing Lane each, with three races of our one presents. The sequence for the bar code are two for the CD and sequences and Alwyn. Illumina Lane info. You could press all three fast too profound. The team match, Pakistan complication by the Stars Delaware using today does not require that. I was online file to perform the analysis. So we only have we only need these for our one for the cell, barcode on our two, for the

city. And I and what's this tutorial? Use the dating site stop samples from the store sells to contain only 15 South instead of a thousand. He have also, for the heritage of the data to only increased the rates on, chromosome 21. So that's the real execution of our, in our can be done within 2 minutes. Over here. And the barcode on Armistice and Fowler check against doesn't own selves. I can see all the to assign specific specific and their design is such a manner that this works with no chance to place in the reference genome.

And now we also use the hg19 version of the human genome and is therefore also need to use the hd19 GTA V channel to Fox TV made in For the indexing real. We are basically trying to reproduce the Galaxy pipeline for single-cell. RNA sequencing data, preprocessing, produce account Matrix from the Fescue and the droplets. You choose to produce a high-quality, trees from Beecher and selling a house before this to Spanish. We have also added a one-time index instead of just being our Workshop. RCW out. We can easily ride together to send Pappas programmatic.

Are we have previews and tested more than a hundred of Jews and pipelines, which can be load of the Divine Define a creditor and Saturdays. So I asked you, but you wasn't time flies, I'll steps we be done in our and then we can get the results right away. That user-specific directory. Here, I show you that using the car functions. first, let me just run this cold chunk to library this to, I could use some, I'll run this couch and also because it will take about one minute.

I'll go back to explain. The CW. CW, update function sinks, the currents are some recipes and returned about, see if they'll cash objects which contains the most updated our stuff. So if you are just using a specific set of tools, you can just run this one time. You don't need to run this. Every time you use the same to you about. If you want to try something new, you chose that they were just added to the collection. You may need to run this off. Artsy number to update if you want to just Mutual

Spell. Okay. Now we got the tools available here and by praying it, it shows about see if they'll cash and then just about to get the local cache of your RCW. Allrecipes 129 records for the existing tools and 5 * and we can use the BFG info out shoes here. It's returned some stuff out cash. Table contains all the information about each of what about your past 9. So it has a colony called our names are named to install it and there's three time. Exercise time I pass the local

Cash Cab. You can also use please to install it starts at 3, our time has passed The tab isn't a planet, or is it a tool? Okay, let's go back over here. So if you starts with a p, l it is a pipeline. If he starts with a t, i v e. How many we have here. So we have a hundred. Three 2026 pipeline. Next, we can't use multiple keywords to search for any specific tools or pipelines of Interest, which internal research that he was against multiple columns in the back table. So let's just search the star and

index. And present 18th update of me while we are interested. So we are just our name of this and used to function. CW store to Source, Darcy. If you are working environment, they have a new object over here and we can also use the ice path. To do the sourcing and let's take a look at the starting next subject so it is star and it shows some requirements. So you don't necessarily have the tools installed but you don't need to worry about it. It's ready. Just run these tool. If you

call, if you pulled the dog current image of the star, the tool on Ryan successfully. So, there are some arguments if it is for the remote and the input is the one that users need to pay more attention to. So, there are four input parameters by Scott Gina with directory. So there's a default value for that. You're a training name and there's Run, try. There's a default value of 4 so because I know how many you want to run. It's probably and there is another

argument called genome data files and sjbg Castile. And there's an output section. That is pretty fun, which vehicle out the input genome directory? As the output switches could be passed as the input for the next step. Okay, now we have a successful, you are and we can rent. So before the line on the Tuesday, in the bath mention the Galaxy view, add an index in style using the tool. Starting next to come online for indexing. Looks like this. If you're an engineer to my life, give me a

call star and the rum old. There's some / they're /. It ran through and the coast of Florida and the folder name for the outputs and you need to pull the absolute. You need to pull the past that have genome over here and need to put the fat house for it. Should have stopped over here. So you think of starting next week so you do the same thing within our reach work, internally passing ICC. They are Suites. What we need to do. It's a sign Wireless for the input parameters and ask you to see the bells great using the rancid that I

function. They also have some haps functions that help you to return some specific information for that are real chill. For example, the Russian you returned the stuff out of this song to the input, the return, all the input parameters that you need your, our users input output h, b, o clock. The directory of genome drive three could be passed as an equal to the next at the requirements Adele carpool. I'm right here. Okay? Now that's a sign by news for this

Prancer. As we have all this input values, you just assign the only one that asked for a step-by-step. So we sign this, Do after a stunning, let me see the info so we can see if there is, I did why they offer this argument. And there's, I did, why do for this argument over here? So as I just mentioned, the rusty, duck internally run. I suggested that I asked for it, so I said no. But if you're interested, we can use this function called, rest to write out a script. So, we can have an idea about

how those internal things are running, so and it is in the file path. Let's go back to the tree and let me go to the outside. So they're too fast generated. Why is Cubs? So it has a very similar case. Our our object, he has a basic information for the class and is Canal all the requirements and the arguments and input. So it has more structured or Matt Stringham put that name prefix, separate equals to 2 minutes. After you read this unit up by space and your input. Put your file path

after that. So the prime turn the sign while it was our separate and the default. Why do we start in there? So there's the default lighting and also in the Yeah, I'm about its 6. All the foul printer Wireless for each parameter. We have the default value over here. We have the assigned, while you for this chinampas Taphouse, we have the same body for the GTA V. And they have the rest riding postal for, okay? BRB have combined both of the, they have combined, both of the configuration

file and why am I into one object? Okay, now, let's run this code. You need two spaces by the two named over here, and you need to speech by. I'll put you right away. So it, maybe I'll post as the and I'll pass out starting next Outpost. They should be generated over here. Let me just run this. so attention, if you are using Cloud these pens, You mean students that dog heart equals to Singularity into false? Otherwise you won't have it, you won't have it run correctly. If you are using

the dog her, you can just leave it and changed. So it is raining right now. Then, we can take a look at the log over here. Wow, it is running out. Explain more about the Run cwl Dog. Her arguments. If you're working on the HPC and the run time, you stopped her images for the required amount. I chose you don't need to pre-install. Any juice on your HTC, also use false, if you work on your local computer. So in this instance we have praised out everything and it's that's not supposed to run times, do you have to change 18 to a false if you want to run this

monthly? And also we have options, how the singularity a string And if you were here on HPC and the run time, you single r&t, you need to use the singularity. In the meantime, you can take a look at the starting decks Bridge, so just as I have shown in the slice, you just need to speechify the inputs crimes. We have 4in footprint directory, it had the type who is streaming the prefix default value. In the second trimester is impossible to help me file, for fakes, no, default value, and the stb, no,

default value and Roth, write an integer prefix, lady called my nigs do for, and I'll put you just read that. You write 3 as an outpost. And then we can construct the tool, using the word Prime. But please find out the requirements and arguments with a default values and input and output. Okay, now we have it. 10 is a size and we can take a look at the output. So we have this folder. If you are still in the house dirko there you can see the new folder generated out starting next year. And if you still have all the applesauce

good for the next step. Okay, so to align the Single Cell race, you can start, it can be done directly income online like this. So a long line for the canal. So in these tests are the come online tool and solo time. There's something Falls, while it was for the solo time and there's the solo, you are my future. Ain't the default value so much time? Do you need to specify the genome directory, which it is the output of the previous in texting. And the solo salbakos watch list

the parking lot. Next over here and refile seeing you. Require those some pasta fell, ask you, if Alfred Florida, South Park, o e r, Suggest a suitable install. And now we should have eight available over here. Let's take a look at these two, so it's the bigger tool. Basic information of the basic commands that bad where Shannon and the requirements for darker and some arguments with the default. Printers and ink put, this will need users to assign values for each of the printers over here and although there will be multiple outputs speak loud and

nice vehicle. The the spam file after the alliance and foul of the log files, and the soda out of the directory of files that include Dallas, Texas, So, I have you a sign. -4 inch of this parameter. Now, let's take a look at the store again, so not in the Inglewood section. We have. the file path for the Reeds house in for the CDMA ask, if I'll let me have the file to pass over here for the house, for the South and maybe a reading from the Previous output run

starting decks tool and the white slave states reading from the East older and there's some default while he was waiting and change, okay? Now, we are ready to run this, so this will be specified out your X-ray to be startled. Output will be generated over of cir. again, if you are running with this, on the college in days till false, They are using Docker keep his pension. selfish me running for About two minutes. I'll go to the scripts here and give you a quick look of the star so long. So, how is refute? So we just define

the five, the Stanley impact, wrenches, the LCM for 60, and a, without seeing for style box from the previous texting stuff to watch list the solo time with a default value. And so you and I love you and drive with a default value of 1. So we Define out of the four multiples between the pattern of them logs star and his Temple and the directory of solo that out. And then late, they seem to give the Constructor with a base. Come out the requirements, the arguments

You take those parameters with default while it was that do you don't need to assign each time an input Prime and Ultra Prime. Nickelback, If you have any questions or you take one, Okay, maybe we can take one question. So, does our cwl expect all third-party software on the pot? Not. So I'll just say it again. So it might have been a question. They asked why you were presenting earlier but So, does our cwl? Expect all third-party software on the father doesn't support any environment. Managers to install dependencies for example conduct for python

package on perhaps a singularity containers with the Lowrance software pre-installed. Address to interfere here was my question. I'm giving you. I don't know if you can see me. And yeah, so she let you answer that question because from what I can see it since we know the software in a doctor of Singularity container, which is fantastic. So I don't know, maybe I can rephrase the question and saying, do you support other containers? Then ducking Singularity, do you for for

software or is that the main source? They're the main source of software third party for the requirements of the other kind of soap do. I just don't like you said it. Also supported you coming after you comment on a computer or you are. So he's why you thought the software from Panda. That's another way to it right now. And thank you. Okay, let me go back to the last session. Now we have a new folder generated should be over here, start solo output of the

Clay County is so they're all his files and in this folder soda out and raw, we have all the, traces we still annotation for the Broncos and features. Okay, let's go to the next step. Now we have all the sales generated in the stars of all post folder and which can be passing through the next tool. Actually, this folder will be patched the solo. All three be passed to the next to a cop chocolate YouTube to get a haircut at 6. We multiply the droplets YouTube tour, which

will produce a future dating sites that is more representative of the syringer pack. My little sister doesn't support the integration of art functions. This is a unique feature for our cwl, we are weak and easy to connect the app stream data previous Arguments for the outputs house. Thanks ready to be wrapped as an artist diabel to, for executions, for example, and using the package droplets, you choose to show you. So we basically threatened so we just write everything into one function, using the job that you choose function. The first thing is to use the

written accounts to read that. The Outpost house from the alignment as it pulled over here on the convert, 18 to a single cell experiment object in our and then we calculated Barthel ranks, and the media, the plot of the brothel ranks and I'll put in the diagnostic PDF and be calculated and teach our place and ploughed this empty chocolate and I'll put in the Diagnostics PDF. Let me have also featured a single cell experiment that you only keeping those real selves. I respect that our data as we

have this function, all ready and we can construct it on our stuff out, you using those double prong. So we need to Define all the three parameters in prancer's into Alpha. Printers one, go out the PDF, file one glove to our data and the construct us without tool. Now we used to do the install and to install, this fell in style this tool into a recession and me. Take a look at the impulse, which we request 3 in post, so many minutes, remaining 43 prime interest and we can run this cold over here. So this will take a little bit of time but less than a

minute. I tweet generator output in the outs dir called. Okay, it's done. So it's raining so size. It should be the jaw play to you too, soft food, and we can take a look at the Diagnostics PDF. So the first sees the Broncos ranked and the second slot is empty job, please. If you are interested in the job that you do, so you can just refer to that, we need to have this package in the workshop now. So we have this. Are they test date? Also, we have these are data saved and you can load it into our and take a quick look.

So, let's peek and see the Single Cell. Experiment, is not only include 10,000 7:15, so it has filtered out size panties. So is the three examples about their hope. You are for me there, I'll for using those existing tools and Jean, the single-celled pre-processing step-by-step and alternately and more easily. We can connect those tools and make a pipeline, which is already available in our stuff. So, I just use the same thing about you stall to Source the pipeline over here, and we can see the plot of your pipeline actually over here. So, if these squares represent the five

vowels, and the Diamonds Are the tools, we have star solo and jock, itch YouTube. This a Third Coast Outpost owls have time for a star, stolen Solo, solo from Star solo. Anime have single size firm until Potenza, are they tight? And the the diagnostic plug from Joppa to YouTube. assign values, for the whole pipeline, the five simple Styles, and we need to run this code for now because each of you, take out It combines the star solo and droplets YouTube so it will take two to three minutes. So in the meantime, you can go to take a look at

the pipeline. Is pretty simple for constructing a pipeline because of me to have already have the Stars solo tool Define, undulate you to use Define. So we Define all the input parameters for the 5B boys, basketball schedule. For CBS Saban coaching at Sunrise, right? Alameda find the overall output for the whole pipeline. So we want to I'll pull the star solo sample and estar solo, spell and I'm even go out and single cell experiment and our data and the Chipettes YouTube Diagnostics

So it is running. I would like to make some additional comments. There are some other cool functionalities that we haven't included in this workshop. For example, the Run. See that we have. That function is the sign for execution of Saddam. Appliance in high-performance Computing big supporter of different jobs Dominion systems, which we beat you with a lot. If you want to run the city of y'all are on your headphones on his computer, and also the open, so user-friendly interface for any age of two packages because Jackson eating

an ice cube thing that schools. And for the previously come online to us, as far as customized our function and the preview, two of them have hands are having multiple arrests and optimized for easy, customizations, more specifics, dates and honest business. And this package, feature request issue, reports and also be welcome for Is importantly, we are trying to make these projects at a community effort, for developing on the sharing of specific set of drawers and pipelines in the back to your

own time for my pistol made. So we look forward to any collaboration singing the plants and please feel free to make your request about Flo from the night National Center for advancing translational science. Okay, let's go back to your x-rays. There's to be icy. I suppose we can't have specified when they flick the run to the bell. I forgot to mention. If you're still running now we have the Four Tops. Miles from the experimental data, the alliance and fell and still owe the directory from the Stars below

intensive. This Workshop, we are happy to take more questions. Thank you. So that's great. It was a very informative Workshop. I'd like to promote Leo who asked a couple of questions to just ask them, unmute himself and just ask it might be easiest and I've been mediating them out later. Question first, so can you specify specific developing apps on a pipeline bullet point? And it seemed to me that fine, maybe the latest version of but maybe you need to use like a specific.

The best one question. Yeah. Okay. Has been I can show them where to put that version of the two. You can Define embark on 1000. Cahaba. They have different version of the trust. You can t find different version of tortilla. You recommend making your own daughter image? Or would you kind of like when you're building your own for your own pipeline? I need to talk to Samsung customer your own script to wrap. You're not at work so you can do your own image. I think that's it. That's one way to customize your clothes. So actually, I was same question, but

someone else ask my plane. Crashes. You run. The same command again, or will it start from an intermediate step? Yeah, that's all I'm going to go to class in. Actually that's a long ways that was that kind of language, you know, they have a motion has 1.2 About. My first question is kind of related to question from earlier which is when you're building a pipeline. So annoying. Are file for start so low right now. And one of the lines said, like that require doctor looks to me like your car coating your I plan to use Docker

but like all your pipeline skip. This one thing and strippers separates that or you can specify where you're using Docker or using. Elmar for environment 103, using my password. So let's say you want to have your script and use it in multiple locations, you need to have multiple. Ours, or you change the change, the requirements, or how would you have? Yeah. A little, until whatever you want the past, when you how to launder to us locally and I already installed on an

estate of the option to go to Forest Lawn. Now, the best, did you go to the store? So you can actually buy the tour. The United Hospital to walk and talk like the first to do that. And the main idea of the book for language is try to solve this soft, a requirement and programs of rain in car in Cars. Eat your to your phone call. I like a user needs to know our cewl. You don't have to take it out to the guys. Thank you very much. So we have time for maybe one more question, and I see one here, if your pipeline crashes,

do you done the same cwl command again, and we'll start from none, Connecticut. Yeah, they all just asked that question. So can you meet Jenny pass for the doctor images that you using say, like stalker image has to have something before example? Like the back of neck image, that sometimes changes, you know, one week is not available next week, so I don't know if you thought about, you need test on the doctors sign. Rapunzel test strips test to check on the

chickens out to us and also we will plan to make a test of their said to us. So we need to get high But can I just add that, isn't it? More ideal to use the release version because it's stable for pipelines and such because it doesn't change almost ever. Yan today for the scripture for the washing, which I could check its Texas weather. I think that's all the time we have so we give chance and seeing a big hand. Thank you so much, nitash.

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